Project partners

University of Milan

University of Milan



The research of Department of Biomedical Sciences and Technologies is in the areas of biochemistry, immunology and new technologies applied to human samples and animal models in the field of physiology and pathology.

The instrumentation and facilities span proteomics, high throughput sequence facilities cell sorting and cytofluorimetry. There is a strong commitment to collaboration with other academic and research institutions.

University of Milan website

Contact: Cecilia Gelfi
Email Cecilia

LITA Building - Via Fratelli Cervi
93 - 20090 Segrate
Milano
Italy
Tel: +39 (0)2 21717 503
Fax: +39 (0)2 21717 558

 

University of Milan and BIO-NMD

The University of Milan will be concerned with muscle proteome differential analysis by 2D-Dige in a selected number of patients and in animal models. They will mostly involved with WP3 - Proteomic biomarkers discovery by studies on patient cells, muscle tissues and body fluids; and WP5 - Biomarker discovery and validation on animal models.

 

Cecilia Gelfi



Cecilia Gelfi is associated Professor of Clinical Biochemistry and Molecular Biology at the University of Milan. She is the coordinator of the Italian Human Proteome Network.

Research activity:

Muscle proteome signature under physiological paraphysiological and pathological conditions.

Brief Outline of research accomplishments:

Active participation in the development of isoelectric focusing in immobilized pH gradients.

Development of a novel thermal theory for predicting with accuracy the real temperature inside the capillary during an electrophoretic run. This has allowed the setting up of a novel technique: separation of DNA fragments, carrying a point mutation for the screening of genetic diseases. Separation of PCR amplified fragments carrying a point mutation cannot be curried out in conventional electrophoresis; the mutants can only be resolved in an electrophoretic run in the presence of a thermal gradient along the separation axis. Since it was impossible to obtain in CZE a thermal gradient in space, we have developed thermal gradients in time.

Active participation in the improvement of methods related to DNA screening particularly for point mutation detection for the diagnosis of genetic defects.

Development of proteomic studies on skeletal muscle taking advantage of the knowledge on the separation science of macromolecules. In a first attempt the interest was focused on the systematic identification of protein in muscle tissue. A large database of proteins localized in high resolution 2D maps and identified by mass spectrometry has been constructed.

At present, new methodologies based on liquid chromatography and mass spectrometry are under developement for the differential quantitation of high MW muscle protein, such as myosin heavy chain, titin, nebulin etc. that play a crucial role in the muscle function but so far are poorly characterized.

New projects are in progress involving differential proteome assessement applyed to specific physiological and physiopathological problems (e.g. hypoxia adaptation, muscle impairments linked to genetic disease, effect of micro gravity, ageing etc.).

 

 

Agnese Vigano

Agnese Vigano is involved in the development of methods for point mutation detections using capillary electrophoresis and all separative technologies of macromolecules, particularly 2D. She is a post-doc at the University of Milan with 14 papers and 10 posters to national and international congress.

 

Sara De Palma

Sara De Palma is a post-doc at the University of Milan and Milano Bicocca. Her experrise is in 2D field and off-gel technologies on protein extracts from small biopsies focused on hypoxia adaptation and dyspherinopathies. She has 10 papers and 8 posters to national and international congress.

 

Daniele Capitanio

Daniele Capitanio is a post-doc at the University of Milan with 10 papers and 11 communications. Her expertise is in muscle molecular characterization by 2D and blue native/SDS. Her main interest is in the data analysis and mining of proteomic differential expression which she applies to FSHD and ALS in animal models.

 

Manuela Moriggi

Manuela Moriggi is a post-doc at the University of Milan with 4 papers and 4 communications. Her expertise are in atrophy invesigated by 2D-DIGE and signalling molecules. Her studies were conducted on human samples after bed rest.

 
 




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